Mechanistic and Synthetic Enzymology
The Yew lab has always been equally fascinated by the astonishing variety of mechanisms by which different enzymes accomplish their mission and the endless possibilities of utilizing enzymes to realize our vision of Planetary Health. We constantly strive to decipher the mechanistic mysteries of enzymes and tailor their sustainable use for Planetary Wellness.
Structural and Mechanistic
Structural and Mechanistic Enzymology
To establish a well-defined structure-function relationship of enzymes and gain a deeper understanding of the mechanisms of enzymatic reactions, we routinely characterize the enzymes under study with a myriad of biochemical, biophysical, and computational tools. Our established structural (X-ray crystallography, cryoEM) and computational (in silico docking, molecular dynamics simulation) biology platforms not only provide deeper insights into enzyme mechanism, but also practical guidance to enzyme design and optimization.
Synthetic Enzymology Tools
Synthetic Enzymology Tools Development
To facilitate the deployment of enzymes into a harsh environment, we are developing protein scaffolds that can be used to immobilize or encapsulate enzymes, lending the enzymes extra support or shield to prolong their lifespan. Such scaffolds can also bring together different enzymes involved in the same reaction cascade, making the overall catalysis more efficient.
Next-Gen Genome Editing
Next-Gen Genome Editing Tools
As a simple, programmable method, the CRISPR-Cas system holds great promise for genome editing of target organisms with unprecedented speed and precision. The relatively large molecular weight and low thermostability of the Cas enzymes are some of the current roadblocks to the wider application of the system. By combining genome data mining with semi-rational design and directed evolution, we are en route to identifying novel Cas enzymes as well as improving existing ones that are more compact and thermostable. Such characteristics will make the Cas enzymes more industrially applicable.